mouse monoclonal antibodies anti ceacam 1  (Santa Cruz Biotechnology)

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: The expression of CEACAM1 on different TSCC groups and its relationship with clinicopathologic features.

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: The relationship of CEACAM1 expression and density of neutrophils.

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: Univariate and Multivariate survival analysis in TSCC patients.

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: Primer sequences and size of PCR products.

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques:

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: A: There were more CD15+ neutrophils (black arrow) in TSCC tissues (b, c, d) than in peritumoral tissues (a). In tumors areas, some neutrophils lied in the stroma of tumors (b), some were located within the carcinoma nests (c), and some infiltrated in the borderline of tumor invasion (d). (a: 100×; b, c, d: 400×). B: The expression of CEACAM1 in peritumoral tissues was negative or weak (a). In TSCC tissues, CEACAM1 expression was obviously stronger than that in peritumoral tissues and located mainly in the cytoplasm of tumor cells (b). (a: 100×; b: 400×). C: The relationship of CEACAM1 expression on tumor cells and infiltration of neutrophils. In strong CEACAM1 expression tumors, there were more neutrophils (black arrow) (a); While in negative or weak CEACAM1 expression tumors, there were relatively fewer neutrophils (b). (a, b: 200×, for CEACAM1).

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: A: The relationship between cumulative cancer-related survival of patients with resectable TSCC and density of neutrophils in tumor tissues. Patients with high density of neutrophils in tumors had significantly reduced cancer-related survival compared to low neutrophils density group ( P = 0.020, Log-rank test). B: The relationship between cumulative cancer-related survival of patients with resectable TSCC and CEACAM1 expression on tumor cells. Patients with moderate or strong expression of CEACAM1 had significantly reduced cancer-related survival compared to negative or weak CEACAM1 expression group ( P = 0.032, Log-rank test).

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Neutrophils Infiltration in the Tongue Squamous Cell Carcinoma and Its Correlation with CEACAM1 Expression on Tumor Cells

doi: 10.1371/journal.pone.0089991

Figure Lengend Snippet: A: Fluorescence microscopic observation of Lv transfection efficientcy. a: CC1-4L-Lv group; b: CC1-4S-Lv group; c: Vector-Lv group. (a, b, c 200×). B: qRT- PCR results of Lv transfection. The results showed that CEACAM1-4L mRNA expression was obviously higher in CC1-4L-Lv transfection group than the other three groups (a), and CEACAM1-4S mRNA expression was the same as 4L (b). C: Western blot results for Lv transfection. a: β-actin; b: CEACAM1-4L (the above band) and -4S (the lower band). Results showed that CEACAM1-4L protein expression was obviously stronger in CC1-4L-Lv transfection group than the other three groups. CEACAM1-4S protein expression was the same as 4L. (Note: CEACAM1-4L protein was slightly bigger than CEACAM1-4S).

Article Snippet: The membrane was blocked with 5% non-fat milk for 1 h, and incubated with monoclonal mouse anti-human CEACAM1 (dilution 1∶500, RD; MAB2244, USA) antibodies for 2 h at room temperature, followed by washing with TBST (TBS containing 0.1% Tween 20) for 5 min five times.

Techniques: Fluorescence, Transfection, Plasmid Preparation, Quantitative RT-PCR, Expressing, Western Blot